单细胞测序是一种下一代测序方法,可通过揭示单细胞分辨率下的遗传表达来深入表征生物分子。它在检测罕见细胞类型、发现肿瘤异质性、痕量谱系、发现耐药细胞群以及表征外周血免疫细胞等复杂细胞群方面优于常规批量测序。Q2 利用领先的技术,如 10x Genomics® Chromium™ 和 Illumina® NovaSeq™ 系统平台。我们可结合我们的全球实验室网络和专家支持团队,提供涵盖临床研究连续体的广泛服务,满足您的测序需求。
使用我们的单细胞 RNA 测序测定,每个样本可测定多达 10,000 个细胞。我们的测定使用 Chromium Controller™ 微流控芯片平台提供 3ʹ 或 5' 技术数字基因表达。将冻存样本解冻并制备成单细胞悬液,然后载入 Chromium Controller™。
使用我们的免疫库测定,从单细胞处理中收集更多的信息。靶向全长配对 T 细胞受体或 B 细胞免疫球蛋白(Ig)转录本,包括同种型和 T 细胞受体 α/β。
我们集中化的科学运营监督与我们的全球实验室布局相结合,可提供全面、大规模的试验支持。从您的临床研究医院到我们的一个中心实验室之间的快速交付,对于保持样本的完整性至关重要。我们可以在 24-48 小时内从世界上几乎任何地方运输样本。
Request a quote today to discover how single cell sequencing with Q2 Solutions can advance your research.
Our global central laboratory network enables testing at a single site.
Obtain in-depth data to understand gene expression, cell surface markers, immune cell repertoire, and much more.
我们的测定方法将单个细胞分配至纳升凝胶珠中进行逆转录并打上细胞条形码,然后进行样本库制备和测序,从而获得单细胞分辨率。我们经验证的样本处理程序可确保可靠的监管链。
我们使用冷冻 PBMC 自动化温控解冻法谨慎地解冻您的样本,降低了操作员差异、过度解冻和污染的风险。我们还利用高通量、自动细胞计数和层流式细胞清洗方案减少差异,提高保留率和活力。
每个细胞被分配至纳米升级凝胶微球乳化液(GEM)中,并打上了独特的条形码,允许对单个细胞进行标记和谱分析。多个样本库可由单个样本构建,生成可链接回同一单细胞的多个读数。
在 Illumina® NovaSeq™ 对制备好的样本库可以进行测序,以便对每个细胞进行高通量、快速和深度测序。
稳健的计算架构、成熟的标准分析渠道和专门的生物信息学家团队能够快速分析样本。应要求,经验丰富的数据分析团队可就您的研究目标提供咨询,并深入探索项目数据。
Webinar: Enabling blood sample prep for single cell sequencing in clinical research
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基因组学数据见解分析解决方案
自定义实验室分析和自定义分析设计
全球下一代测序 (Next Generation Sequencing, NGS) 服务
基因表达和分析服务,检测可归因于 38 种关键免疫细胞亚型、个体基因和比率的免疫特征谱
独特的高参数流式细胞术服务和可定制解决方案,旨在满足全球范围内的临床试验需求
整个产品开发生命周期中优化生物标志物策略的专业知识、经验以及工具
我们基于 DNA 的 T 细胞受体 βeta (TCRβ) 测序测定法可通过以 10-6 的灵敏度测量克隆动态和探询克隆种群来全面表征适应性免疫应答
Clinical Genomics Laboratory Services
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Single cell RNA sequencing (scRNA-seq) uncovers tissue heterogeneity by providing gene expression measurements in individual cells within a tissue. A current limitation of scRNA-seq is the...
Next generation sequencing (NGS)-based comprehensive genomic profiling has emerged as a powerful tool for the large-scale detection of genomic changes. TruSight Oncology 500 (TSO500) Assay employs a...
Download this presentation to learn more about a new protocol using Chromium Gene Expression Flex to successfully and reliably conduct single cell RNA sequencing with FFPE tumor biopsy tissues. ...