Single Cell RNA Sequencing
Accelerating research by exploring cellular heterogeneity, tracking population changes, and uncovering molecular interactions
10x Certified Service Provider
Single Cell RNA Sequencing
Accelerating research by exploring cellular heterogeneity, tracking population changes, and uncovering molecular interactions
10x Certified Service Provider
Uncovering Cellular Diversity to Advance Clinical and Translational Research
Single cell sequencing is a next-generation sequencing method enabling in-depth characterization of biomolecules by uncovering genetic expression at single cell resolution. It has advantages over conventional bulk sequencing in its ability to detect rare cell types, uncover tumor heterogeneity, trace lineages, discover drug-resistant cell populations, and characterize complex cell populations such as immune cells in peripheral blood. Q2 Solutions utilizes leading technologies such as the 10x Genomics® Chromium™ and Illumina® NovaSeq™ platforms. We can meet your sequencing needs with our extensive services spanning the clinical research continuum combined with our global laboratory network and expert support team.
High Quality, High Throughput, High Satisfaction
Customizable Single Cell Sequencing Solutions to Meet Your Research Needs
Single Cell RNA-Seq
Profile up to 10,000 cells per sample with our single cell RNA sequencing assay. Our assay provides 3ʹ or 5' digital gene expression using the Chromium Controller™ microfluidic platform. Cryopreserved samples are thawed and prepared into single cell suspensions before loading into the Chromium Controller™.
Single Cell Immune Repertoire – TCR & BCR Profiling
Expand on the information gathered from single cell processing with our immune repertoire assay. Target full-length paired T-cell receptor or B-cell immunoglobulin (Ig) transcripts, including isotypes and T-cell receptor α/β.
Single Cell Surface Protein Profiling
Incorporate cell surface protein detection and antigen specificity analysis in conjunction with gene expression and immune repertoire profiling with our surface protein profiling assay.
Supporting Studies Around the World
Our centralized scientific and operational oversight combined with our global laboratory footprint enables comprehensive, large-scale trial support. Expeditious delivery from your clinical site to one of our central labs is essential for maintaining the integrity of your samples. We can transport samples from almost anywhere in the world within a 24-48hr timeframe.
Sample Preparation & Assessment
We carefully thaw your samples using our automated, temperature-controlled thaws for cryopreserved PBMCs, reducing the risk of operator variability, over thawing, and contamination. We also utilize high-throughput, automated cell counting and laminar flow cell washing protocols to reduce variability and enhance retention and viability.
Library Construction
Each cell is partitioned into nanoliter-scale Gel Beads-in-Emulsion (GEMs) and uniquely barcoded, allowing for labeling and profiling of individual cells. Multiple libraries can be constructed from a single sample, generating multiple readouts that can be linked back to the same single cell.
Sequencing
Prepared libraries are sequenced on Illumina® NovaSeq™ to enable high throughput, rapid, and deep sequencing for each cell.
Bioinformatics Analysis and Delivery
Rapid analysis of samples is enabled by robust computational architecture, a mature standard analysis pipeline, and a dedicated team of bioinformaticists. Upon request, an experienced data analytics team is available to consult on your research objectives and enable deep exploration of project data.
