Single cell sequencing is a next-generation sequencing method enabling in-depth characterization of biomolecules by uncovering genetic expression at single cell resolution. It has advantages over conventional bulk sequencing in its ability to detect rare cell types, uncover tumor heterogeneity, trace lineages, discover drug-resistant cell populations, and characterize complex cell populations such as immune cells in peripheral blood. Q2 utilizes leading technologies such as the 10x Genomics® Chromium™ and Illumina® NovaSeq™ platforms. We can meet your sequencing needs with our extensive services spanning the clinical research continuum combined with our global laboratory network and expert support team.
Profile up to 10,000 cells per sample with our single cell RNA sequencing assay. Our assay provides 3ʹ or 5' digital gene expression using the Chromium Controller™ microfluidic platform. Cryopreserved samples are thawed and prepared into single cell suspensions before loading into the Chromium Controller™.
Expand on the information gathered from single cell processing with our immune repertoire assay. Target full-length paired T-cell receptor or B-cell immunoglobulin (Ig) transcripts, including isotypes and T-cell receptor α/β.
Incorporate cell surface protein detection and antigen specificity analysis in conjunction with gene expression and immune repertoire profiling with our surface protein profiling assay.
Our centralized scientific and operational oversight combined with our global laboratory footprint enables comprehensive, large-scale trial support. Expeditious delivery from your clinical site to one of our central labs is essential for maintaining the integrity of your samples. We can transport samples from almost anywhere in the world within a 24-48hr timeframe.
Request a quote today to discover how single cell sequencing with Q2 Solutions can advance your research.
Our global central laboratory network enables testing at a single site.
Obtain in-depth data to understand gene expression, cell surface markers, immune cell repertoire, and much more.
Our assay acquires single cell resolution by partitioning individual cells into nano-liter gel beads where they undergo reverse transcription and cellular barcoding followed by library preparation and sequencing. Our validated sample handling procedures ensure reliable chain of custody.
We carefully thaw your samples using our automated, temperature-controlled thaws for cryopreserved PBMCs, reducing the risk of operator variability, over thawing, and contamination. We also utilize high-throughput, automated cell counting and laminar flow cell washing protocols to reduce variability and enhance retention and viability.
Each cell is partitioned into nanoliter-scale Gel Beads-in-Emulsion (GEMs) and uniquely barcoded, allowing for labeling and profiling of individual cells. Multiple libraries can be constructed from a single sample, generating multiple readouts that can be linked back to the same single cell.
Prepared libraries are sequenced on Illumina® NovaSeq™ to enable high throughput, rapid, and deep sequencing for each cell.
Rapid analysis of samples is enabled by robust computational architecture, a mature standard analysis pipeline, and a dedicated team of bioinformaticists. Upon request, an experienced data analytics team is available to consult on your research objectives and enable deep exploration of project data.
Webinar: Enabling blood sample prep for single cell sequencing in clinical research
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Analysis solutions for insights from genomic data
Custom laboratory assay and custom analysis design
Global Next Generation Sequencing (NGS) Services
Gene expression and analysis services for the detection of Immune Landscape Signatures attributable to 38 key immune cell subtypes, individual genes, and ratios
Unique high parameter flow cytometry services implemented with customizable solutions are designed to meet clinical trial needs across the globe
Expertise, experience, and tools for an optimized biomarker strategy throughout your product’s development life cycle
Our DNA based T-cell receptor βeta (TCRβ) sequencing assay allows for a comprehensive characterization of the adaptive immune response through measurement of clonal dynamics and interrogation of clonal populations to a sensitivity of 10-6
Clinical Genomics Laboratory Services
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