Q2 Header_1280x350-single-cell

Single-Cell RNA Sequencing

Accelerating research by exploring cellular heterogeneity, tracking population changes, and uncovering molecular interactions

10x Certified Service Provider

Uncovering Cellular Diversity to Advance Clinical and Translational Research

Single-cell sequencing is a next-generation sequencing method enabling in-depth characterization of biomolecules by uncovering genetic expression at single-cell resolution. It has advantages over conventional bulk sequencing in its ability to detect rare cell types, uncover tumor heterogeneity, trace lineages, discover drug-resistant cell populations, and characterize complex cell populations such as immune cells in peripheral blood. Q2 utilizes leading technologies such as the 10x Genomics® Chromium™ and Illumina® NovaSeq™ platforms. We can meet your sequencing needs with our extensive services spanning the clinical research continuum combined with our global laboratory network and expert support team.

t-sne-projection-cells-clustering

High Quality, High Throughput, High Satisfaction

 

image3

 

Customizable Single-Cell Sequencing Solutions to Meet Your Research Needs


scs6

Single-Cell RNA-Seq

Profile up to 10,000 cells per sample with our single-cell RNA sequencing assay. Our assay provides 3ʹ or 5' digital gene expression using the Chromium Controller™ microfluidic platform. Cryopreserved samples are thawed and prepared into single-cell suspensions before loading into the Chromium Controller™.

scs5

Single-Cell Immune Repertoire – TCR & BCR Profiling

Expand on the information gathered from single-cell processing with our immune repertoire assay. Target full-length paired T-cell receptor or B-cell immunoglobulin (Ig) transcripts, including isotypes and T-cell receptor α/β.

scs7

Single-Cell Surface Protein Profiling

Incorporate cell surface protein detection and antigen specificity analysis in conjunction with gene expression and immune repertoire profiling with our surface protein profiling assay.

scs8

Single-Nucleus RNA-Seq

Interrogate difficult to dissociate cell types and uncover complex heterogeneity with our single-nucleus assay. Use fresh frozen tissue to faithfully capture RNA expression by circumventing transcriptional changes induced by traditional dissociation.

Request a Quote

Supporting Studies Around the World

Our centralized scientific and operational oversight combined with our global laboratory footprint enables comprehensive, large-scale trial support. Expeditious delivery from your clinical site to one of our central labs is essential for maintaining the integrity of your samples. We can transport samples from almost anywhere in the world within a 24-48hr timeframe.

global-reach-single-cell-sequencing

Simplify Your Work

Simplify Your Work
Request a Quote

Request a quote today to discover how single-cell sequencing with Q2 Solutions can advance your research.

Ship Your Samples

Our global central laboratory network enables testing at a single site.

Get Your Results

Obtain in-depth data to understand gene expression, cell surface markers, immune cell repertoire, and much more.

Technology You Can Trust

Our assay acquires single-cell resolution by partitioning individual cells into nano-liter gel beads where they undergo reverse transcription and cellular barcoding followed by library preparation and sequencing. Our validated sample handling procedures ensure reliable chain of custody.

samples
Sample Preparation & Assessment

We carefully thaw your samples using our automated, temperature-controlled thaws for cryopreserved PBMCs, reducing the risk of operator variability, over thawing, and contamination. We also utilize high-throughput, automated cell counting and laminar flow cell washing protocols to reduce variability and enhance retention and viability.

barcode-specimen
Library Construction

Each cell is partitioned into nanoliter-scale Gel Beads-in-Emulsion (GEMs) and uniquely barcoded, allowing for labeling and profiling of individual cells. Multiple libraries can be constructed from a single sample, generating multiple readouts that can be linked back to the same single-cell.

sequencing
Sequencing

Prepared libraries are sequenced on Illumina® NovaSeq™ to enable high throughput, rapid, and deep sequencing for each cell.

clipboard-data
Bioinformatics Analysis and Delivery

Rapid analysis of samples is enabled by robust computational architecture, a mature standard analysis pipeline, and a dedicated team of bioinformaticists. Upon request, an experienced data analytics team is available to consult on your research objectives and enable deep exploration of project data.

Frequently Asked Questions

What is single-cell sequencing?
Single-cell sequencing is a next generation sequencing method involving single-cell isolation, transcriptome characterization, and sequencing library generation. Single-cell sequencing uncovers heterogeneity at a high resolution by revealing cellular gene expression, quantity, and diversity in a sample.
What advantages does single-cell sequencing have over traditional methods?
The main difference between single-cell sequencing and traditional methods of bulk sequencing, qPCR, and flow cytometry is that single-cell sequencing allows for characterization of heterogenous samples by profiling the whole transcriptome at a single-cell resolution. Our 96-throughput library preparation capability is a 12-fold increase over traditional methods. Single-cell is often used in conjunction with traditional methods to obtain a comprehensive understanding of samples.
What controls do you have in place to limit variability?
We have automated various methods in our workflow to decrease variability. We automate temperature-controlled thaws to replace manual water baths, high-throughput cell counting to obtain objective percent viability and counting, and laminar flow cell washing to reduce manipulation and stress from traditional centrifugation methods. Additionally, we remove dead cells which, if left unaddressed, cause imperfect results and missed sequencing targets.
What data do I receive at the end of the experiment?
Core Deliverables
Per Sample Files
  • FASTQ
  • BAM
  • 10x HTML QC
  • Cluster CSVs indicating cluster for each cell
  • Differential expression CSVs corresponding to each cluster CSV
  • Principal Components Analysis CSVs
  • tSNE projections CSV for the coordinates of each cell
  • Raw and filtered feature barcode matrices containing cell UMI counts and gene annotation files (H5 and MTX)
  • cloupe file for visualization and analysis in Loupe browser
  • Compressed h5 files for use in different analysis programs
  • Table containing one record per cellular barcode providing a prediction of dead cell status, multiplet status
Batch files
  • EA Genomics Web Summary Report: contains summary of sequencing, alignment, estimated cells, dead cells, multiplets, and human cell type frequencies annotated using SingleR (exploratory)
TCR/BCR Data Files
  • Heavy and Light Chain Clonotypes (BCR)
  • Paired Clonotypes (TCR)
  • Contig and Consensus sequences
  • Metrics Summary
Can I customize the assay to fit my specific research needs?
Yes! We understand that no two research projects are exactly alike. We work with you to understand your particular research needs, and our team provides cost-effective recommendations to accomplish your goals. Contact our team today for more information about custom assay development and specialized bioinformatics support.
Where can I find answers to other frequently asked questions?
You can find additional answers on our General Genomics FAQ webpage.
Request a Quote

Learn More


Related Products

Bioinformatics Services

Analysis solutions for insights from genomic data

Custom Assay Development

Custom laboratory assay and custom analysis design

Next Generation Sequencing Services

Global Next Generation Sequencing (NGS) Services

Immune Landscape Signatures

Gene expression and analysis services for the detection of Immune Landscape Signatures attributable to 38 key immune cell subtypes, individual genes, and ratios

Flow Cytometry Services

Unique high parameter flow cytometry services implemented with customizable solutions are designed to meet clinical trial needs across the globe

Biomarker Services

Expertise, experience, and tools for an optimized biomarker strategy throughout your product’s development life cycle

TCR Immune Repertoire Sequencing

Our DNA based T-cell receptor βeta (TCRβ) sequencing assay allows for a comprehensive characterization of the adaptive immune response through measurement of clonal dynamics and interrogation of clonal populations to a sensitivity of 10-6

Related Services & Solutions


Related Thought Leaders Insights


The potential of applying Flow Cytometry as an IVD technology for CDx

In this episode, Megan McCausland, Scientific Advisor for Flow Cytometry at Q2 Solutions, and Scott Bornheimer, Associate Director of Medical and Scientific Affairs at BD Biosciences, discuss the...

Translational Science and Innovation Laboratory (TSAIL)

Located in the heart of the Research Triangle Park, Q2 Solutions’ state-of-the-art Translational Science and Innovation Laboratory (TSAIL) is designed for exploratory biomarker research and new...

Evaluation and Reproducibility Study using NanoString GeoMx Immune Pathway Panel

The rapidly advancing field of spatial biology has led to development of new platforms which provide measurements of gene and protein expression and their precise localization to defined tissue...