960 x 350_41

SARS-CoV-2 Viral Genome NGS Testing

A highly sensitive targeted amplicon NGS solution facilitating comprehensive profiling of the SARS-CoV-2 genome

Complete genome characterization of SARS-CoV-2 is instrumental in the understanding of the COVID-19 pandemic. The emergence of variants of concern has placed a greater importance in monitoring changes to the SARS-CoV-2 genome through NGS. Gain a deeper understanding of underlying influences affecting vaccine and therapeutic efficacy by monitoring changes in the viral genome over time with our SARS-CoV-2 viral genome assay. We utilize the Ion Ampliseq SARS-CoV-2 Research Panel, a targeted amplicon sequencing chemistry, to provide complete genome coverage and accurate variant calling at low viral loads allowing for flexibility to a range of sample types. This panel consists of 2 primer pools providing redundant coverage minimizing any potential coverage dropout as a result of mutational changes in the viral genome. Using a comprehensive data analysis solution, we are able to deliver a complete SARS-CoV-2 viral genome sequence with accurate variant calling and informed annotation.

 

NGS Workflow

 

  Nasopharyngeal Swab
  • NGS assay designed to test wide range of viral loads
  • On-going development expanding acceptable sample types
      
  Viral Load Quantitation
  • Quantitative SARS-CoV-2 Viral Load Assay
  • NGS Library optimized based on viral load  
  Viral RNA Isolation
  • High-Throughput Automated Isolation
  • Magnetic bead based viral RNA isolation protocol
  Ion Genestudio S5 Sequencing
  • High-Throughput Ion AmpliSeq Technology
  • Complete viral genome coverage with great sensitivity

 

 

Thermo Fisher Ion Ampliseq SARS-CoV-2 Research Panel

Reference sequence: MN908947.3

Covers >99% of the SARS-CoV-2 genome, all serotypes included

100% Accuracy and Sensitivity for SNVs and Indels above LoD

Data Deliverables:

  • Assembled SAR-CoV-2 Viral Genome (FASTA)
  • Variant Calls against viral reference genome (VCF)
  • Annotated Variants (TSV)
medical-data-on-screen

Related Thought Leaders Insights


Quantitation of Replication Competent Viruses using ddPCR

Retroviral and lentiviral vectors are increasingly being used in the engineering of oncologic cell therapies; however, they may pose risk to human health in the event the viral vector becomes...

Rapid turnaround Oncomine Precision Assay enables global harmonization for clinical trial testing

Genetic variation in neoplasia can take the form of single or multiple nucleotide variants, gene fusions, amplifications, and/or splice variations. This heterogeneity can pose a challenge in...

Innovation Laboratories

Q2 Solutions Innovation Laboratories is located in the heart of the dynamic Research Triangle Park and within our global headquarters in Durham, NC, USA. This facility creates an innovative...