Microsatellite instability (MSI) results from an accumulation of insertion or deletion of repeating units during DNA replication in tumor cells with a deficient mismatch repair (MMR) system. MSI status is associated with response/resistance to certain immune checkpoint inhibitors. Q2 Solutions MSI assay utilizes multiplex, fluorescent PCR and capillary electrophoresis to enable sensitive detection of five mononucleotide repeat markers (BAT-25, BAT-26, NR-21, NR-24 and MONO-27) in tumor FFPE specimens. Microsatellite instability at two or more mononucleotide loci is interpreted as MSI-High; microsatellite instability at a single mononucleotide locus is interpreted as MSI-Low; no instability at any of the loci tested is interpreted as microsatellite stable (MSS).
Example of Colon Cancer FFPE Tissue Specimen Demonstrating MSI-High Status
Example capillary electropherogram demonstrating reference, normal profile in normal tissue (upper panel) and MSI-High profile in tumor FFPE tissue (lower panel). New MSI events labeled 1−5 are detected in tumor tissue.
MSI Assay Specifications | |
---|---|
Markers | BAT-25, BAT-26, MONO-27, NR-21, and NR-24 microsatellite loci |
Specimen Requirements | Tumor: 3 x 5um FFPE tumor slides or 3 curls or FFPE block or DNA. Minimum 20% neoplastic cellularity required. Normal: either whole blood (1-3 mL, K2EDTA) or tumor FFPE slides with clearly indicated normal tissue area or 3 x 5um normal tissue FFPE slides, curls or block. If H&E stained slide with indicated tumor normal areas is not available, will perform H&E assessment and determine specimen adequacy. |
Assay Method | OncoMate® MSI Dx Analysis System |
System Compatibility | Thermo Fisher Scientific SeqStudio Flex |
Regulatory Tier | RUO, GCP, CAP-CLIA |
Deliverables | MSI Status report. Exact number of MSI markers available as custom report. |
As a leading laboratory services organization for trials across the globe, we integrate therapeutic insights, state-of-the-art technologies, best-in-class methods and quality systems to optimize your...
Visium spatial profiling is a next-generation sequencing method that enables the analysis of cellular relationships by combining transcriptomics with histological techniques. It has advantages over...
This brochure highlights the specialty immunoassays performed by Rules-Based Medicine, a Q2 Solutions company. RBM provides an extensive menu of quantitative protein biomarkers, including multiplexed...