TMB Standardization by Alignment to Reference Standards

Phase 2 of the Friends of Cancer Research TMB Harmonization Project

At ASCO 2019, we presented a poster with Friends of Cancer Research (FOCR), highlighting their Tumor Mutational Burden (TMB) Harmonization Project. The goal of this Project is to harmonize TMB estimates through alignment to reference standards in order to improve consistency across panels, for the sake of optimizing clinical application and facilitating integration of datasets generated from multiple assays.

In Phase 1 of the TMB Harmonization Project, a robust correlation was demonstrated between TMB estimated using targeted NGS gene panels and whole exome sequencing (WES) applied to TCGA data. These findings demonstrated the theoretical variability in TMB estimates across panels. Phase 2 employs sustainable TMB reference standard materials to assess the empirical variability in TMB estimates.

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