Evaluation of Single Cell RNA Sequencing from FFPE Tissue using 10x Genomics Chromium Gene Expression Flex Assay
Single cell RNA sequencing (scRNA-seq) uncovers tissue heterogeneity by providing gene expression measurements in individual cells within a tissue. A current limitation of scRNA-seq is the requirement to isolate viable cells from fresh or cryopreserved solid tumor biopsies, especially given that formalin-fixed paraffin embedded (FFPE) materials are ubiquitously utilized for long-term tissue preservation. Tissue fixation fragments genetic material, which introduces challenges for RNA-seq methods due to the reduced size of template material.
The objective of this study was to describe and characterize the utilization of single cells prepared from FFPE tissue sections to establish sample requirements, key quality control metrics, and reproducibility of the 10x Genomics Gene Expression Flex workflow. Download poster to learn more.
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