Liposomal Encapsulated and Free Topotecan Analysis in Human Tissues, Plasma, Ultrafiltrate, and Urine by Liquid Chromatography/Tandem Mass Spectrometry
LC/MS/MS analysis of total and free topotecan in multiple matrices across a broad concentration range following liposomal topotecan administration provides a complete picture of drug delivery
This work quantified free form topotecan in human plasma, plasma ultrafiltrate, urine, and tissue homogenate samples from patients dosed with liposomal formulated topotecan. Each matrix had individualized sensitivity, stability, and sample handling requirements to preserve the free and liposomal forms during collection and analysis.
An interdependent workflow approach was taken for each method to be optimized in tandem.
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