Protein Characterization

We provide a customized approach to ensure successful generation of high-quality proteins with strong lot-to-lot reproducibility. We integrated characterization through a project, instead of waiting until the end. This custom approach helps us provide rapid orthogonal evaluation of protein-related key parameters. We’re constantly implementing new assays and adapting existing ones for specific applications.

Protein concentration

• Colorimetric

• (Bradford/Lowry/BCA/RCDC)

• Absorbance at 280nm

Aggregation

• SEC-HPLC

• SEC-MALS

• Analytical ultracentrifugation (SV-AUC)

• Dynamic Light Scattering (DLS)

• SDS-PAGE

Reactivity

• ELISA

We also offer orthogonal evaluation of related key parameters:

• Purity – N-term/C-term homogeneity, chemical stability, post-translational modifications, residual protease identification

• Size in solution – oligomerization state, aggregation susceptibility, resistance to stress

• Stability and folding – low and high order structure, thermodynamic evaluation, force degradation study

• Sequence integrity – N-term/C-term homogeneity, chemical stability, post-translational modifications

• Biomolecular interaction – rate of association/dissociation

Characterization Services Menu

Our protein sciences team offers a range of analytical methods that can be used to characterize a broad range of proteins and antibodies.