A Process of Method Development for Tissue Homogenization and Analysis to Eliminate Binding and Matrix Effects Using Surrogate Matrices
The analysis and distribution of pharmaceuticals in tissue is increasingly important in understanding the full action of drugs in vivo. Across molecular types from very small molecules to larger peptides and oligonucleotides, bioanalysis of tissues presents unique challenges inherent quantitative bioanalysis in a mixed matrix assay.
- Describes a method of processing to mitigate binding and suppression effects in tissue homogenates
- Demonstrate surrogate curves are effective at compensating for matrix effects in multiple matrices
- Demonstrate increased workflow efficiency and acceptable assay performance using a single surrogate curve for multiple tissues.