Scientific Poster
Victor Weigman, Ph.D. | June 10, 2019

TMB Standardization by Alignment to Reference Standards

Phase 2 of the Friends of Cancer Research TMB Harmonization Project
At ASCO 2019, we presented a poster with Friends of Cancer Research (FOCR), highlighting their Tumor Mutational Burden (TMB) Harmonization Project. The goal of this Project is to harmonize TMB estimates through alignment to reference standards in order to improve consistency across panels, for the sake of optimizing clinical application and facilitating integration of datasets generated from multiple assays.

In Phase 1 of the TMB Harmonization Project, a robust correlation was demonstrated between TMB estimated using targeted NGS gene panels and whole exome sequencing (WES) applied to TCGA data. These findings demonstrated the theoretical variability in TMB estimates across panels. Phase 2 employs sustainable TMB reference standard materials to assess the empirical variability in TMB estimates.

Download poster to learn more.

Related Services:
Tumor Mutational Burden (TMB) Assay for Response to Immunotherapy

TMB assay utilizing whole exome sequencing (WES) methodology and proprietary variant analysis compendium

Panel-based tumor mutational burden (TMB) analysis of matched tumor and plasma specimens using Illumina’s TruSight Oncology 500 next-generation sequencing assay

AACR 2019 Scientific Poster

Genomic Based Solutions for Your Immuno-oncology Drug Development Needs

Genomics-based biomarker approach

T-cell Receptor (TCR) Sequencing Assay for Characterization of Adaptive Immune Response

Detects TCRβ and TCRγ clonotypes expressed at the mRNA level

Read More