Using Cyclic Nucleotides to Assess Endogenous Dilution Accuracy as a Measure of Biomarker Selectivity in LC/MS/MS

Accurate measurements of cAMP and cGMP as a useful biomarkers for development of therapeutics for a variety of indications

Download our scientific poster to learn about the measurement of the endogenous analytes adenosine 3′,5′-cyclic monophosphate (cAMP) and guanosine 3′,5′-cyclic monophosphate (cGMP) via a surrogate matrix approach.  A method was developed that demonstrated excellent statistical results; however, during validation, dilution of unfortified control plasma with surrogate matrix showed a positive bias for cGMP but not cAMP. An increase in cGMP mean endogenous level was also observed. The cause of this positive bias was an occasional co-eluting interferent present in ~20% of samples. Addition of trifluoroacetic acid (TFA) to the mobile phase solutions allowed for baseline resolution of the interferent from cGMP. 

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Authors: John E. Buckholz, Barry R. Jones, Raymond F. Biondolillo, Robert M. Sturm, Philip E. Joyce, Danielle J. Strong, John R. Perkins, Jennifer M. Cox and Bradley L. Ackermann

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