Bottom-Up Quantitative Measurement of a Candidate Antibody Therapeutic in Human Plasma by LC-MS/MS using Formic Acid Digestion

Presented at the 2016 American Society for Mass Spectroscopy Sanibel Conference, this presentation highlights how Q2 Solutions developed a liquid chromatography with tandem mass spectrometry (LC-MS/MS) assay for quantification of a candidate antibody therapeutic in human plasma. Using formic acid digestion, the Q2 Solutions team obtained good assay statistics, including inter-individual selectivity and accuracy.

Q2 Solutions is one of the few bioanalytical laboratories successfully employing immunoprecipitation techniques and multi-dimensional LC for the quantitation of biotherapeutics, peptides, proteins, and biomarkers. Our team has the experience and knowledge in development and validation of small and mid-size peptides to proteins, fusion proteins and monoclonal antibodies. The development of these assays is followed by method validation and sample analysis consistent with global regulatory requirements and governed by SOPs.

Download the presentation to learn more about LC-MS/MS method development at Q2 Solutions.

Jack Henion, Ph.D., Vice President, R&D
Barry Jones, Ph.D., Associate Director, LCMS Biologics and Biomarkers