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Validated bioanalytical methods list

Advanced methods to deliver consistent, reliable data

Q2 Solutions operates one of the world’s largest and most respected bioanalytical laboratory networks. From 20+ years of experience we have developed numerous robust methods used to deliver the vital data you need. Beyond those listed below we can also develop customized methods to meet your specific challenge.

LC/MS/MS Methods

Compound/Metabolite Range Extraction
Volume
Matrix/Anticoagulant
Acyclovir; Valaciclovir 10 – 1000 ng/mL 50 μL Human plasma/K3EDTA
Amlodipine 0.05 – 5 ng/mL 20 μL Human plasma/K2EDTA
D-Amphetamine; L-Amphetamine (chiral) 0.5 – 75 ng/mL 200 μL Human plasma/sodium EDTA
Amphetamine (achiral) 0.5 – 200 ng/mL 100 μL Human plasma/sodium EDTA
Aprepitant 10 – 10,000 ng/mL 50 μL Human plasma/K2EDTA
Asenapine and Desmethylasenapine 0.02 – 20 ng/mL 200 μL Human plasma/K2EDTA
Atorvastatin; o-Hydroxyatorvastatin; p-Hydroxyatorvastatin 0.25 – 25 ng/mL 200 μL Human plasma/sodium heparin and K3EDTA
Bortezomib 0.1 – 25 ng/mL 50 or 100 μL Human plasma/K2EDTA, K3EDTA, and sodium heparin
Available soon
Budesonide/
Formoterol
 
2 – 1000 pg/mL
0.2 – 100 pg/mL
 
500 μL
 
Human plasma/K2EDTA
Bupropion; Hydroxybupropion 0.1 – 100 ng/mL 100 μL Human plasma/K2EDTA
Caffeine; Paraxanthine 20 – 20,000 ng/mL 100 μL Human plasma/K2EDTA (surrogate matrix Bovine Serum
Albumin (BSA) Phosphate Buffered Saline (PBS))
Capecitabine; 5'-DFCR/
5'-DFUR/
5-FU/
FBAL
10 – 5000 ng/mL
50 – 25,000 ng/mL
2 – 1000 ng/mL
15 – 7500 ng/mL
200 μL Human plasma/sodium EDTA and K3EDTA
Capecitabine/
5-Fluorouracil
10 – 5000 ng/mL
2 – 1000 ng/mL
200 μL Human plasma/K3EDTA
Carbamazepine/
Carbamazepine 10,11-epoxide
20 – 5000 ng/mL
20 – 500 ng/mL
50 μL Human plasma/sodium EDTA
Clopidogrel/
Clopidogrel Acid/
Clopidogrel Active Metabolite (H4 diastereomer)
0.05 – 25 ng/mL
5 – 5000 ng/mL
0.05 – 50 ng/mL
100 μL Human plasma/K2EDTA
Clopidogrel Active Metabolite (H4 diastereomer only) 0.0706 – 70.6 ng/mL 200 μL Human plasma/K2EDTA
Cyclosporine A 1 – 1000 ng/mL 20 μL Human blood/K2EDTA
Cytarabine 25 – 1000 ng/mL 20 μL Human plasma/sodium heparin
Cytarabine/
Ara-Uridine
0.5 – 1000 ng/mL
2 – 1000 ng/mL
100 μL Human plasma/K2EDTA
Cytarabine/
Ara-Uridine
20 – 20,000 ng/mL
50 – 50,000 ng/mL
100 μL Human plasma/K2EDTA
Danazol 0.5 – 500 ng/mL 100 μL Human plasma/K2EDT
Desipramine/2-Hydroxydesipramine 0.25 – 100 ng/mL 20 μL Human plasma/K2EDTA
Daunorubicin; Daunorubicinol 1 – 500 ng/mL 100 μL Human plasma/K2EDTA
Decitabine 0.5 – 500 ng/mL 50 μL Human plasma/K2EDTA
Dextromethorphan/
Dextrorphan
0.01 – 5 ng/mL
0.5 – 250 ng/mL
100 μL
 
Human plasma/K2EDTA
 
Digoxin
 
0.05 – 5 ng/mL
 
200 μL
 
Human serum
Human plasma/K2EDTA
Digoxin 0.1 – 20 ng/mL 200 μL Human serum
Digoxin 1 – 200 ng/mL 200 μL Human urine
Digoxin 5 – 1000 ng/mL 100 μL Human urine
Docetaxel 1 – 2500 ng/mL 200 μL Human plasma/K2EDTA
Donepezil 0.2 – 100 ng/mL 200 μL Human plasma/sodium heparin
Available Soon
Doxorubicin
 
1 – 500 ng/mL
 
50 μL
 
Human plasma/K2EDTA
Duloxetine/
Atomoxetine; S,S-Reboxetine
0.2 – 200 ng/mL
0.5 – 500 ng/mL
50 μL Human plasma/K3EDTA
Entecavir 25 – 15,000 pg/mL 1000 μL Human urine
Entecavir 25 – 20,000 pg/mL 200 μL Human plasma/K2EDTA
Available Soon
Epinephrine
 
0.5 – 10 ng/mL
 
100 μL
 
Human plasma/K2EDTA
Erlotinib 1 – 3000 ng/mL 50 μL Human plasma/sodium EDTA
Available Soon
Estriol
 
5 – 5000 pg/mL
 
500 μL
 
Human plasma/K2EDTA
5-Fluorouracil (5-FU) 2 – 10,000 ng/mL 100 μL Human plasma/sodium EDTA
5-Fluorouracil (5-FU) 10 – 10,000 ng/mL 100 μL Human plasma/sodium EDTA and K2EDTA
Fenofi bric Acid 20 – 10000 ng/mL 100 μL Human plasma/sodium EDTA
Fluconazole 10 – 10,000 ng/mL 20 μL Human plasma/K2EDTA
Available Soon
Flurbiprofen
 
TBD
 
TBD
 
TBD
Available Soon
Formoterol/
Budesonide
 
0.2 – 100 pg/mL
2 – 1000 pg/mL
 
500 μL
 
Human plasma/K2EDTA
Available Soon
Fluticazone/Salmeterol
 
5 – 200 pg/mL
 
200 μL
 
Human plasma/K2EDTA
Gemcitabine 10 – 10,000 ng/mL 25 μL Human plasma/lithium heparin
Gemcitabine; 2☒,2☒-Difluorodeoxyuridine 10 – 10,000 ng/mL 20 μL Human plasma/lithium heparin
Glyburide (total) 1 – 250 ng/mL 200 μL Human plasma/sodium heparin
Imatinib/
N-Desmethyl Imatinib
100 – 10,000 ng/mL
50 – 5000 ng/mL
50 μL Human plasma/K2EDTA
Itraconazole; Hydroxy Itraconazole 1 – 1000 ng/mL 20 μL Human plasma/K2EDTA
Ketoconazole 10 – 10,000 ng/mL 20 μL Human plasma/K2EDTA
Lamivudine (as part of Combivir®) 2 – 1000 ng/mL 200 μL Human plasma/sodium heparin
Lamivudine (as part of Combivir®) 2 – 1000 ng/mL 200 μL Human urine
Lovastatin; Lovastatin Acid 0.25 – 100 ng/mL 500 μL Human plasma/sodium heparin
Lovastatin; Lovastatin Acid 0.25 – 125 ng/mL 100 μL Human plasma/K2EDTA
Lumefantrine 50 – 20,000 ng/mL 50 μL Human plasma/K2EDTA
Metformin 10 – 2000 ng/mL 20 μL Human plasma/sodium heparin
Methotrexate (Low Range)/
7-Hydroxymethotrexate
0.25 – 200 ng/mL
1 – 120 ng/mL
200 μL Human plasma/lithium heparin and K2EDTA
Methotrexate (Low Range)/
7-Hydroxymethotrexate
1 – 1000 ng/mL
1 – 150 ng/mL
200 μL Human plasma/lithium heparin and K2EDTA
Methotrexate 0.25 – 200 ng/mL 200 μL Human plasma/K2EDTA
Methotrexate (Low Curve) 50 – 1000 ng/mL 20 μL Human urine
Methotrexate (High Curve)/
7-Hydroxymethotrexate
1000 – 50,000 ng/mL
50 – 1000 ng/mL
20 μL Human urine
Methotrexate (Low Curve)/
7-Hydroxymethotrexate
50 – 10,000 ng/mL
20 – 1000 ng/mL
20 μL Human urine
Methylphenidate; α-Phenyl-α-(2-Piperidyl)Acetic Acid 0.05 – 25 ng/mL 100 μL Human plasma/acid citrate dextrose
Metoprolol; α-Hydroxymetoprolol 0.500 – 250 ng/mL 100 μL Human plasma/K2EDTA
Midazolam; 1ʹ-Hydroxymidazolam 0.1 – 100 ng/mL 100 μL Human plasma/K2EDTA
Midazolam; 1ʹ-Hydroxymidazolam 0.1 – 100 ng/mL 20 μL Human plasma/sodium and lithium heparin
Montelukast 2 – 2000 ng/mL 20 μL Human plasma/sodium heparin and K2EDTA
Available Soon
Montelukast/36-Hydroxy Montelukast
 
2 – 2000 ng/mL
 
20 μL
 
Human plasma/K2EDTA
Moxifloxacin 25 – 5000 ng/mL 50 μL Human plasma/K2EDTA, K3EDTA, sodium EDTA,
sodium heparin, and lithium heparin
Nisoldipine 0.02 – 20 ng/mL 100 μL Human plasma/K2EDTA
Available Soon
Norethindrone
 
50 – 12,500 pg/mL
 
500 μL
 
Human plasma/K2EDTA
Omeprazole/
5ʹ-Hydroxyomeprazole; Omeprazole Sulfone
1 – 500 ng/mL
0.5 – 250 ng/mL
20 μL Human plasma/K2EDTA and sodium heparin
Paclitaxel; 6-Hydroxypaclitaxel 10 – 5000 ng/mL 200 μL Human plasma/K2EDTA
Paliperidone 0.100 – 100 ng/mL 100 μL Human plasma/K2EDTA
Paroxetine 0.025 – 25 ng/mL 50 μL Human plasma/K2EDTA
Pemetrexed 10 – 5000 ng/mL 50 μL Human plasma/K2EDTA
Available Soon
Phenytoin
 
20 – 20,000 ng/mL
 
20 μL
 
Human plasma/K2EDTA
Repaglinide 0.01 – 20 ng/mL 50 μL Human plasma/sodium heparin
Rifampicin (Rifampin) 10 – 5000 ng/mL 100 μL Human plasma/K2EDTA
Risperidone 0.1 – 100 ng/mL 50 μL Human plasma/K2EDTA
Ritonavir 10 – 5000 ng/mL 50 μL Human plasma/K3EDTA
Rivastigmine 0.1 – 30 ng/mL 100 μL Human plasma/potassium
oxalate/sodium fluoride
Rosiglitazone 5 – 3000 ng/mL 25 μL Human plasma/K3EDTA
Rosuvastatin/
Rosuvastatin Lactone; N-Desmethyl Rosuvastatin
0.5 – 100 ng/mL
0.5 – 50 ng/mL
100 μL Human plasma/K2EDTA
Available Soon
Saquinavir
 
1 – 1000 ng/mL
 
100 μL
 
Human plasma/K2EDTA
Sildenafil 1 – 1000 ng/mL 50 μL Human plasma/K2EDTA
Sildenafil; N-Desmethyl Sildenafil 1 – 1000 ng/mL 50 μL Human plasma/K2EDTA
Simvastatin; Simvastatin Acid 0.1 – 30 ng/mL 200 μL Human plasma/K3EDTA
Sunitinib/
N-Desmethyl Sunitinib
0.1 – 60 ng/mL
0.1 – 20 ng/mL
20 μL Human plasma/K2EDTA
Telaprevir 50 – 20,000 ng/mL 100 μL Human plasma/K2EDTA
Tenofovir 2 – 1000 ng/mL 100 μL Human plasma/K2EDTA
Tolbutamide; 4-Hydroxytolbutamide 50 – 50,000 ng/mL 20 μL Human plasma/K2EDTA
Tolterodine; 5-Hydroxymethyl Tolterodine 0.2 – 50 ng/mL 100 μL Human plasma/sodium heparin
Valproic Acid 1 – 100 μg/mL 100 μL Human serum
Verapamil; Norverapamil 0.2 – 200 ng/mL 50 μL Human plasma/K2EDTA
R-Warfarin; S-Warfarin (chiral) 0.1 – 10 ng/mL 250 μL Human PFF (non-protein bound)
R-Warfarin; S-Warfarin (chiral) 1 – 2500 ng/mL 100 μL Human plasma (total)/sodium heparin
Zidovudine (as part of Combivir®) 2 – 1000 ng/mL 200 μL Human plasma/sodium heparin
Zidovudine (as part of Combivir®) 2 – 1000 ng/mL 200 μL Human urine
Ziprasidone 0.5 – 150 ng/mL 200 μL Human plasma/sodium heparin


Immunoassay Biomarker Methods

Biomarker/
Analyte on ELISA platform
Quantitation Range Assay
Volume
Minimum Required
Dilution (MRD)
Matrix
HER-2/neu 2.5 – 35 ng/mL 10 μL 1:50 Human serum
β-NGF (free) 1 – 500 pg/mL 25 μL 1:1 (no MRD) Human serum
IGF-1 (total) 0.15 – 6 ng/mL 20 μL 1:100 (treated sample) Human serum
IGF-1 (free) 0.15 – 6 ng/mL 10 μL 1:50 (untreated sample) Human serum
sECAD 0.625 – 20 ng/mL 20 μL 1:20 Human serum
sEGFr 12.5 – 300 ng/mL 10 μL 1:50 Human serum
VEGF 50 – 1000 pg/mL 100 μL 1:2 Human plasma/sodium citrate


LC/MS Biomarker Methods

Biomarker Method Development and Validation Expertise
Amino Acids, Lipids, Neurotransmitters, Nucleotides, Hormones, Peptides, Proteins – immunoprecipitation, digestion, immunoaffinity LC/LC/nano LC/MS/MS
Biomarker Non-Proprietary Methods
Biomarker Range Volume Required Matrix
Amino Acid Panel 0.5 – 100 μg/mL 50 μL Human plasma/K2EDTA
4β-Hydroxycholesterol 2 – 300 ng/mL 50 μL Human plasma/K2EDTA
β-Nerve Growth Factor (NGF) 10 – 1280 pg/mL 200 µL Human serum
Cholesterol 0.75 – 3.5 mg/mL 10 µL Human plasma/K2EDTA
Citrulline 300 – 40,000 ng/mL 20 µL Human plasma/K2EDTA
Digoxin 0.05 – 5 ng/mL 200 µL Human plasma/K2EDTA
7α-Hydroxy-4-cholesten-3-one (C4) 2 – 250 ng/mL 40 μL Human serum
Insulin 40 – 1250 pg/mL 350 μL Human serum
Kynurenine/
Tryptophan
0.05 – 15 μg/mL
0.2 – 60 μg/mL
20 μL Human plasma/K3EDTA
Rat Hepcidin 2 – 500 ng/mL 25 μL Rat serum